New PDF release: Aminergic and Peptidergic Receptors. Satellite Symposium of

By E. S. Vizi

ISBN-10: 0080263852

ISBN-13: 9780080263854

ISBN-10: 0080268390

ISBN-13: 9780080268392

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Additional info for Aminergic and Peptidergic Receptors. Satellite Symposium of the 3rd Congress of the Hungarian Pharmacological Society, Szeged, Hungary, 1979

Example text

Protein were determined by the m e t h o d of L o w r y et al / l l / . RESULTS The brain cyclic AMP 5 58 t h level d e c r e a s e d slowly during larvae stage and reached a m i n i m u m value at the very the beginning of the l a r v a e - a d u l t ecdysis /Fig. 1/. In the of the m o u l t i n g a n i m a l the cyclic AMP level i n c r e a s e d ly and reached a m a x i m u m v a l u e . In the adult a n i m a l gradually decreased sharp- the again. pmol cAMP/mg tissue brain cyclic AMP c o n c e n t r a t i o n brain «— , r——i J ^th ecdysis 2 4 1 6 6 7 i J 1 larvae-adult ecdysis 3 5 7 days F i g .

A n i m a l s at the e c d y s i s and the m o u l t i n g i n d i v i d u a l y u n d e r v i s u a l c o n t r o l of the cultures. The b r a i n s w e r e removed by d i s s e c t i o n and extracted by the m e t h o d of G i l m a n / 1 6 / . The c o n c e n t r a t i o n of cyclic was e s t i m a t e d a c c o r d i n g to the d e t a i l e d p r o c e d u r e in the A m e r s h a m outlined cyclic AMP kit. 5 mM EGTA. 1 in a final v o l u m e of 500 jul. for 5 m i n . at 0 ° C , ATP was added to start the r e a c t i o n .

A n i m a l s the s t o m a c h s w e r e r e m o v e d , c o n t a i n i n g M g S 0 4 , E G T A and 0 . 1 % T r i t o n - X - 1 0 0 , c e n t r i f u g e d saline and the m u c o s a l killed 1 hr after t h e last t r e a t m e n t , Biochem. 2C 15/ /ref. 01/. 017 tissue/60 to rats i n t r a p e r i t o n e a l l y 2_2, 2299 , 1 9 7 3 / . HDI w e r e a d m i n i s t e r e d Pharm. 4-imidazolyl-3-amino-2-butanone from c o n t r o l d in p a r e n t h e s e s . 848 - Dose mg/kg i. p . Control Treatment OF HD ADENYLATE CYCLASE ACTIVITY AFTER PROLONGED ADMINISTRATIONS THE H I S T A M I N E - S T I M U L A T E D TABLE 3 o b t a i n e d that in the rat h y p o t h a l a m u s b o t h H^-and H 2~ r e c e p t o r s are p r e s e n t w h i l e in the g a s t r i c m u c o s a o n l y the system is involved in h i s t a m i n e responses.

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Aminergic and Peptidergic Receptors. Satellite Symposium of the 3rd Congress of the Hungarian Pharmacological Society, Szeged, Hungary, 1979 by E. S. Vizi


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